Proteome studies on leaf peroxisomes from Spinacia oleracea L. and Arabidopsis thaliana (L.) Heynh.

TL;DRAbstract

A proteome analysis of leaf peroxisomes was initiated to further increase our understanding of the functions of this ubiquitous eukaryotic subcellular entity. A reported method for the isolation of leaf peroxisomes from Spinacia oleracea L. was improved by inclusion of a second density gradient which reduced the contamination of leaf peroxisomes by chloroplasts and mitochondria to an undetectable minimum, although a novel type of proplastid-like organelle was still present in the peroxisome fractions. In addition, an ingenious method was designed to enable comparative proteome analyses of highly pure spinach leaf peroxisomes from stress-treated plants in a minimal time, allowing the isolation of leaf peroxisomes from two different plant sets in parallel and their direct comparison. To take advantage of the information of the first genome sequence of a higher plant, namely that of Arabidopsis thaliana L., for proteome analyses, a novel efficient procedure for the enrichment of its leaf

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