Reduced and skewed TCR Vβ repertoire in CD8 compared to Foxp3+ regulatory T cells in acute gastrointestinal graft versus host disease (TRAN1P.947)

TL;DRAbstract

Abstract It is unclear if skewing of TCR repertoire in CD4, CD8 and Foxp3+Tregs plays a role in acute gastrointestinal (GI) GVHD. This prospective clinical study examined the TCR Vβ diversity among CD4, CD8 and Foxp3+Tregs from 18 adult patients (median age 53) post allogeneic stem cell transplant (SCT) at day 80 with acute GI GVHD (giGVHD, >grade 1, n=9) vs. nonGVHD (n=9). Fresh PBMC were analyzed by FACS using IOTest Beta Mark 24 TCR Vβ and Foxp3 (236A/E7)/Helios eBioscience kits. Mann Whitney test was used. Mean %CD4 was lower in giGVHD vs. nonGVHD (44vs72%, p=0.02) while no difference in %CD8 (51vs55%), %Tregs (16vs16%) and %Helios+Tregs (72vs76%). %Total Vβ were similar for CD4 (60), CD8 (60) and Tregs (64) in nonGVHD. In giGVHD, %CD8 (40) was lower than %Tregs (69), p<0.001 and %CD4 (56), p=0.08. Between giGVHD vs. nonGVHD, there was a difference for %TCR in CD8 (p=0.02) but none for CD4 or Tregs. In nonGVHD, there was a direct correlation between %CD8 TCR vs. %CD8

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