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Article10.1096/fasebj.24.1_supplement.753.1

The Effects of Cell Lysates of Bifidobacterium longum (BL730) and Lactobacillus casei (E5) on in vitro Stimulation of Mouse Immune Cells

Woo Sik Lee,Seong Gu Hwang,Oliver D. Abanto,Seung‐Yong Park-2010-04-01-The FASEB Journal
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TL;DRAbstract

The present study was conducted to investigate in vitro mechanisms involved in the immunostimulatory effects of Bifidobacterium longum and Lactobacillus casei . RAW 264.7 macrophage cell line, primary cultures of BALB/c peritoneal macrophages and neutrophils were treated with different concentrations of B. longum lysates ( BL ) or L. casei lysates ( LC ). Nitric oxide ( NO ) production and IL‐6 cytokine expression were measured using nitrite assay and sandwich ELISA, respectively. Neutriphils and macrophages were treated with or without BL or LC, and the phagocytotic activities were measured using FACS. The antioxidant activity of BL and LC were analysed using DPPH assay. Both lysates induced NO production in both cell line and primary cultured cells. The NO production in primary cultures of macrophages and neutrophils were significantly higher (p<0.05) when treated with 500 μg/mL of LC than those treated with the same dose of BL. The BL stimulated IL‐6 cytokine expression significa

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The present study was conducted to investigate in vitro mechanisms involved in the immunostimulatory effects of Bifidobacterium longum and Lactobacillus casei . RAW 264.7 macrophage cell line, primary cultures of BALB/c peritoneal macrophages and neutrophils were treated with different concentrations of B. longum lysates ( BL ) or L. casei lysates ( LC ). Nitric oxide ( NO ) production and IL‐6 cytokine expression were measured using nitrite assay and sandwich ELISA, respectively. Neutriphils and macrophages were treated with or without BL or LC, and the phagocytotic activities were measured using FACS. The antioxidant activity of BL and LC were analysed using DPPH assay. Both lysates induced NO production in both cell line and primary cultured cells. The NO production in primary cultures of macrophages and neutrophils were significantly higher (p<0.05) when treated with 500 μg/mL of LC than those treated with the same dose of BL. The BL stimulated IL‐6 cytokine expression significa

Keywords

Bifidobacterium longumLactobacillus caseiImmune systemCytokineIn vitroCell cultureChemistryBifidobacterium

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