Estudo da expressão do Gene ABR em leucemia mieloide cronica (LMC) utilizando real-time RT-PCR (qPCR),

TL;DRAbstract

sensitivity in GeneAmp 5700 Sequence Detector System was tested by amplification with ABR, BCR, ACTB e GAPD primers and resulted in minimal detection of de 1, 12, 10 e 9 molecules, respectively. The normalization strategy adopted in this work was helpful for accurate qPCR profiling, and is especially suitable for studying the relevance of biological signals of low intensities.

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